Clinical R&D

Clinical research

Clonality analysis of various hematopoietic disorders in cats naturally infected with feline leukemia virus. J Vet Med Sci. 2000;62(10):1059-1065.

PCR detection of the Cryptococcus neoformans CAPS9 gene from a biopsy specimen from a case of feline cryptococcosis. J Vet Diagn Invest. 2001 Sep;13(5):439-442.

Hematologic abnormalities and outcome of 16 cats with myelodysplastic syndromes. J Vet Intern Med. 2001;15:471-477.

Assignment of the feline Fas ligand gene (TNFSF6) to chromosome F1q12-->q13 by fluorescence in situ hybridization. Cytogenet Cell Genet. 2001;94(1-2):92-93.

Assignment of the feline Fas (TNFRSF6) gene to chromosome D2p13-->p12.2 by fluorescence in situ hybridization. Cytogenet Cell Genet. 2001;95(1-2):122-124.

Detection of the integrated feline leukemia viruses in a cat lymphoid tumor cell line by fluorescence in situ hybridization. J Hered. 2003 May-Jun;94(3):251-255.

Clinicopathological features and therapy of myelodysplastic syndromes in two dogs. Vet Rec. 2003 Jul 5;153(1):25-27.

Treatment of chronic lymphocytic leukaemia in three dogs with melphalan and prednisolone. J Small Anim Pract. 2004 Jun;45(6):298-303.

Thymoma in a dog with a part of granular cell proliferation and concurrent lymphoma cells. J Vet Med Sci. 2004 Jun;66(6):713-715.

Characterization of a newly established nonproducer lymphoma cell line for feline leukemia virus. Vet Immunol Immunopathol. 2004 Dec 28;102(4):429-439.

In vivo expression of GFP transgene delivered via a replicating feline leukemia virus. Vet Microbiol. 2005 Oct 31;110(3-4):181-195.

Prognostic factors associated with survival in dogs with lymphocytic-plasmacytic enteritis. J Vet Med Sci. 2006 Sep;68(9):929-933.

Essential thrombocythemia in a dog. J Vet Med Sci. 2006 Nov;68(11):1203-1206.

C-reactive protein concentration in canine idiopathic polyarthritis. J Vet Med Sci. 2006 Dec;68(12):1275-1279.

Induction of chemoresistance in a cultured canine cell line by retroviral transduction of the canine multidrug resistance 1 gene (mdr1). Am J Vet Res. 2007 Jan;68(1):95-100.

Application of RNA interference for inhibiting the replication of feline immunodeficiency virus in chronically infected cell lines. Vet Microbiol. 2007 Mar 10;120(3-4):207-216.

Measurement of intestinal mucosal permeability in dogs with lymphocytic-plasmacytic enteritis. J Vet Med Sci. 2007 Jul;69(7):745-749.

C-reactive protein concentration in dogs with various diseases. J Vet Med Sci. 2008 Feb;70(2):127-131.

Molecular pathogenesis of feline leukemia virus-induced malignancies: insertional mutagenesis. Vet Immunol Immunopathol. 2008 May 15;123(1-2):138-143.

Effect of Tyrosine Kinase Inhibition by Imatinib Mesylate on Mast Cell Tumors in Dogs. J Vet Intern Med. 2008 Jul-Aug;22(4):985-988.

Inhibition of the replication of feline immunodeficiency virus by lentiviral vector-mediated RNA interference in feline cell lines. J Vet Med Sci. 2008 Aug;70(8):777-783.

Investigation of various methods for the cryopreservation of canine bone marrow-derived CD34(+) cells. J Vet Med Sci. 2008 Nov;70(11):1211-1217.

Vascular and kupffer imaging of canine liver and spleen using the new contrast agent Sonazoid. J Vet Med Sci. 2008; 70: 1265-1268.

Hypereosinophilic syndrome in two cats. J Vet Med Sci. 2008 Oct;70(10):1085-1089.

Quantitative assessment of minimal residual disease (MRD) in canine lymphoma by using real-time polymerase chain reaction. Vet Immunol Immunopathol. 2008 Dec 15;126(3-4):321-331.

Quantitative analysis of mRNA transcripts of Hox, SHH, PTCH, Wnt, and Fzd genes in canine hematopoietic progenitor cells and various in vitro colonies differentiated from the cells. J Vet Med Sci. 2009 Jan;71(1):69-77.

Inhibitory effect of newly developed CXC-chemokine receptor 4 antagonists on the infection with feline immunodeficiency virus. J Vet Med Sci. 2009 Jan;71(1):121-124.

Reticulated platelet levels in whole blood and platelet-rich plasma of dogs with various platelet counts measured by flow cytometry. J Vet Med Sci. 2009 Feb;71(2):195-197.

Identification of a novel common proviral integration site, flit-1, in feline leukemia virus induced thymic lymphoma. Virology. 2009 Mar 30;386(1):16-22.

Characterization of canine focal liver lesions with contrast-enhanced ultrasound using a novel contrast agent-sonazoid. Vet Radiol Ultrasound. 2009 Mar-Apr;50(2):188-194.

Antiviral activity of membrane fusion inhibitors that target gp40 of the feline immunodeficiency virus envelope protein. Vet Microbiol. 2009 Apr 14;136(1-2):155-159.

Prevalence of hematological abnormalities and detection of infected bone marrow cells in asymptomatic cats with feline immunodeficiency virus infection. Vet Microbiol. 2009 May 12;136(3-4):217-225.

Disseminated histiocytic sarcoma with excessive hemophagocytosis in a cat. J Vet Med Sci. 2009 Jun;71(6):817-820.

Characterization of monocyte-derived dendritic cells from cats infected with feline immunodeficiency virus. J Vet Med Sci. 2009 Jul;71(7):865-871.

Blood hyaluronic acid as a marker for canine cirrhosis. J Vet Med Sci. 2009 Sep;71(9):1251-1254.

Sensitivity for the detection of a clonally rearranged antigen receptor gene in endoscopically obtained biopsy specimens from canine alimentary lymphoma. J Vet Med Sci. 2009 Dec;71(12):1673-1676.

Mutations in the fifth immunoglobulin-like domain of kit are common and potentially sensitive to imatinib mesylate in feline mast cell tumours. Br J Haematol. 2010 Jan;148(1):144-153.

Molecular cytogenetic analysis of feline leukemia virus insertions in cat lymphoid tumor cells. J Virol Methods. 2010 Feb;163(2):344-352.

Evaluation of prognostic factors and establishment of a prognostic scoring system for canine primary immune-mediated hemolytic anemia. J Vet Med Sci. 2010 Apr;72(4):465-470.

Long term survival of primary skeletal muscle lymphoma in a miniature dachshund. J Vet Med Sci. 2010 May;72(5):673-677.

Monitoring of minimal residual disease (MRD) after multidrug chemotherapy and its correlation to outcome in dogs with lymphoma: a proof-of-concept pilot study. J Vet Intern Med. 2010 Jul-Aug;24(4):897-903.

Quantitative analysis of mRNA for 10 different drug resistance factors in dogs with lymphoma. J Vet Med Sci. 2010 Sep;72(9):1165-1172.

Aberrant autophosphorylation of c-Kit receptor in canine mast cell tumor cell lines. Vet Immunol Immunopathol. 2010 Oct 15;137(3-4):208-216.

Eosinophilia and Eosinophilic Infiltration into Splenic B-Cell High-Grade Lymphoma in a Dog. J Vet Med Sci. 2010 Oct;72(10):1367-1370.

Myeloma-related disorder with leukaemic progression in a cat. J Feline Med Surg. 2010 Dec;12(12):982-987.

A Retrospective Study in 21 Shiba Dogs with Chronic Enteropathy. J Vet Med Sci. 2011 Jan;73(1):1-5.

Characterization of canine BCL6 cDNA and detection systems for its protein expression. Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):166-169.

Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma. Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):307-311.

Evaluation of cytoreductive efficacy of vincristine, cyclophosphamide, and Doxorubicin in dogs with lymphoma by measuring the number of neoplastic lymphoid cells with real-time polymerase chain reaction. J Vet Intern Med. 2011 Mar-Apr;25(2):285-291.

Increase in minimal residual disease in peripheral blood before clinical relapse in dogs with lymphoma that achieved complete remission after chemotherapy. J Vet Intern Med. 2011 Mar-Apr;25(2):292-296.

Expression of fibrosis-related genes in canine chronic hepatitis. Vet Pathol. 2011 Jul;48(4):839-845.

A case of recovery from canine destructive cholangitis in a Miniature Dachshund. J Vet Med Sci. 2011 Jul;73(7):937-939.

Comparison between immunohistochemistry and genetic clonality analysis for cellular lineage determination in feline lymphomas. J Vet Med Sci. 2011 Jul;73(7):945-947.

Spontaneous acute erythroblastic leukaemia (AML-M6Er) in a dog. J Small Anim Pract. 2011 Aug;52(8):445-447.

Plasma high-mobility group box 1 (HMGB1) in dogs with various diseases: comparison with C-reactive protein. J Vet Med Sci. 2011 Sep 30;73(9):1127-1132.

Real-time ultrasonographic evaluation of canine gastric motility in the postprandial state. J Vet Med Sci. 2011 Sep 30;73(9):1133-1138.

Multiplex PCR and Genescan analysis to detect immunoglobulin heavy chain gene rearrangement in feline B-cell neoplasms. Vet Immunol Immunopathol. 2011 Sep 15;143(1-2):38-45.

Perfusion method for harvesting bone marrow cells from dogs. Am J Vet Res. 2011 Oct;72(10):1344-1348.

Screening of therapeutic targets for canine mast cell tumors from a variety of kinase molecules. J Vet Med Sci. 2011 Oct;73(10):1295-1302.

Ultrasonographic evaluation of vincristine-induced gastric hypomotility and the prokinetic effect of mosapride in dogs. J Vet Intern Med. 2011 Nov;25(6):1461-1464.

Quantification of chemokine and chemokine receptor gene expression in duodenal mucosa of dogs with inflammatory bowel disease. Vet Immunol Immunopathol. 2011 Dec 15;144(3-4):290-298.

Prokinetic Effect of the 5-HT4R Agonist Mosapride on Canine Gastric Motility. J Vet Med Sci. 2011 Dec;73(12):1635-1637.

Evaluation of the prognostic significance of BCL6 gene expression in canine high-grade B-cell lymphoma. Vet J. 2012 Jan;191(1):108-114.

A Retrospective Study of Inflammatory Colorectal Polyps in Miniature Dachshunds. J Vet Med Sci. 2012 Jan;74(1):59-64.

Molecular cloning and characterization of canine fractalkine and its receptor CX3CR1. Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):100-109.

Molecular cloning and expression analysis of the canine chemokine receptor CCR9. Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):534-549.

GeneScan analysis to detect clonality of T-cell receptor γ gene rearrangement in feline lymphoid neoplasms. Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):402-409.

Decreased gallbladder emptying in dogs with biliary sludge or gallbladder mucocele. Vet Radiol Ultrasound. 2012 Jan-Feb;53(1):84-91.

Biological effect of tyrosine kinase inhibitors on three canine mast cell tumor cell lines with various KIT statuses. J Vet Pharmacol Ther. 2012 Feb;35(1):97-104.

A case of canine multiple inflammatory colorectal polyps treated by endoscopic polypectomy and argon plasma coagulation. J Vet Med Sci. 2012 Apr;74(4):503-506.

CT characteristics of primary hepatic mass lesions in dogs. Vet Radiol Ultrasound. 2012 May-Jun;53(3):252-257.

Comparison of the antitumor effects of an MDM2 inhibitor, nutlin-3, in feline lymphoma cell lines with or without p53 mutation. Vet Immunol Immunopathol. 2012 Jun 30;147(3-4):187-194.

Intestinal T-cell lymphoma with severe hypereosinophilic syndrome in a cat. J Vet Med Sci. 2012 Aug;74(8):1057-1062.

Increased expression of fractalkine and its receptor CX3CR1 in canine inflammatory bowel disease and their possible role in recruitment of intraepithelial lymphocytes. Vet Immunol Immunopathol. 2012 Aug 15;148(3-4):226-235.

Effect of mosapride on prednisolone-induced gastric mucosal injury and gastric-emptying disorder in dog. J Vet Med Sci. 2012 Sep;74(9):1103-1108.

Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease. Vet J. 2012 Oct;194(1):66-70.

X-chromosome inactivation pattern analysis for the assessment of cell clonality in cats. Vet Pathol. 2012 Nov;49(6):963-970.

Serum amyloid A uptake by feline peripheral macrophages. Vet Immunol Immunopathol. 2012 Nov 15;150(1-2):47-52.

Cytomorphological and immunological classification of feline lymphomas: clinicopathological features of 76 cases. J Vet Med Sci. 2013;75(6):701-707.

Analyses on activation of NF-κB and effect of bortezomib in canine neoplastic lymphoid cell lines. J Vet Med Sci. 2013;75(6):727-731.

Simultaneous inactivation of the p16, p15 and p14 genes encoding cyclin-dependent kinase inhibitors in canine T-lymphoid tumor cells. J Vet Med Sci. 2013;75(6):733-742.

Value of the 13C-urea breath test for detection of gastric Helicobacter spp. infection in dogs undergoing endoscopic examination. J Vet Med Sci. 2013;75(8):1049-1054.

Decreased immunoglobulin A concentrations in feces, duodenum, and peripheral blood mononuclear cells of dogs with inflammatory bowel disease. J Vet Intern Med. 2013 Jan-Feb;27(1):47-55.

Two cases of feline orbital aspergillosis due to Aspergillus udagawae and A. viridinutans. J Vet Med Sci. 2013 Jan 31;75(1):7-10.

Lymphangiomatosis of the systemic skin in an old dog. J Vet Med Sci. 2013 Feb;75(2):187-190.

The prognostic significance of minimal residual disease in the early phases of chemotherapy in dogs with high-grade B-cell lymphoma. Vet J. 2013 Mar;195(3):319-324.

Serum amyloid A as a prognostic marker in cats with various diseases. J Vet Diagn Invest. 2013 May;25(3):428-432.

Polypoid adenomas secondary to inflammatory colorectal polyps in 2 miniature dachshunds. J Vet Med Sci. 2013 May 2;75(4):535-538.

Protease-activated receptor-2 induces proinflammatory cytokine and chemokine gene expression in canine keratinocytes. Vet Immunol Immunopathol. 2013 May 15;153(1-2):17-25.

Validation of the prognostic value of histopathological grading or c-kit mutation in canine cutaneous mast cell tumours: a retrospective cohort study. Vet J. 2013 Jun;196(3):492-498.

Regulation of expression of ABCB1 and LRP genes by mitogen-activated protein kinase/extracellular signal-regulated kinase pathway and its role in generation of side population cells in canine lymphoma cell lines. Leuk Lymphoma. 2013 Jun;54(6):1309-1315.

Development and evaluation of a novel in-clinic automated hematology analyzer, ProCyte Dx, for canine erythrocyte indices, leukogram, platelet counts and reticulocyte counts. J Vet Med Sci. 2013 Nov;75(11):1519-1524.

Regulations of ABCB1 and ABCG2 expression through MAPK pathways in acute lymphoblastic leukemia cell lines. Anticancer Res. 2013 Dec;33(12):5317-5323.

The association between gall bladder mucoceles and hyperlipidaemia in dogs: a retrospective case control study. Vet J. 2014 Jan;199(1):76-79.

Epigenetic regulation of the ABCB1 gene in drug-sensitive and drug-resistant lymphoid tumour cell lines obtained from canine patients. Vet J. 2014 Jan;199(1):103-109.

Prognostic significance of the expression levels of the p16, p15, and p14 genes in dogs with high-grade lymphoma. Vet J. 2014 Feb;199(2):236-244.

The regulation of the expression of ABCG2 gene through mitogen-activated protein kinase pathways incanine lymphoid tumor cell lines. J Vet Med Sci. 2014 Mar 1;76(2):237-242.

Plasma transferring concentration as a nutritional marker in malnourished dogs with nutritional treatment. J Vet Med Sci. 2014 Apr;76(4):539-543.

Clinical characteristics and prognostic factors in dogs with histiocytic sarcomas in Japan. J Vet Med Sci. 2014 May;76(5):661-666.

Prognostic analyses on anatomical and morphological classification of feline lymphoma. J Vet Med Sci. 2014 Jun;76(6):807-811.

Phase I dose-escalation study of nimustine in tumor-bearing dogs. J Vet Med Sci. 2014 Jun;76(6):895-899.

Chronic myelogenous leukaemia with persistent neutrophilia, eosinophilia and basophilia in a cat. J Feline Med Surg. 2014 Jun;16(6):517-521.

Antitumour effect and modulation of expression of the ABCB1 gene by perifosine in canine lymphoid tumour cell lines. Vet J. 2014 Jul;201(1):83-90.

Differential expression of CD45 isoforms in canine leukocytes. Vet Immunol Immunopathol. 2014 Jul 15;160(1-2):118-122.

Methylation of TNFRSF13B and TNFRSF13C in duodenal mucosa in canine inflammatory bowel disease and its association with decreased mucosal IgA expression. Vet Immunol Immunopathol. 2014 Jul 15;160(1-2):97-106.

Inhibition of p16 tumor suppressor gene expression via promoter hypermethylation in canine lymphoid tumor cells. Res Vet Sci. 2014 Aug;97(1):60-63.

Intestinal protease-activated receptor-2 and fecal serine protease activity are increased in canine inflammatory bowel disease and may contribute to intestinal cytokine expression. J Vet Med Sci. 2014 Aug;76(8):1119-1127.

Evaluation of DNA methylation profiles of the CpG island of the ABCB1 gene in dogs with lymphoma. Am J Vet Res. 2014 Sep;75(9):835-841.

Hypermethylation of the death-associated protein kinase CpG island in canine B-cell lymphoid tumors. Vet Immunol Immunopathol. 2014 Oct 15;161(3-4):222-231.

Demonstration of the cell clonality in canine hematopoietic tumors by X-chromosome inactivation pattern analysis. Vet Pathol. 2015 Jan;52(1):61-69.

Construction of a multicolor GeneScan analytical system to detect clonal rearrangements ofimmunoglobulin and T cell receptor genes in canine lymphoid tumors. Vet ImmunolImmunopathol. 2015 May 15;165(1-2):81-87.

Evaluationof the drug sensitivity and expression of 16 drug resistance-related genes in canine histiocytic sarcoma cell lines. J Vet Med Sci. 2015 Jun;77(6):677-684.

Detection of circulating tumor cellsusing GeneScan analysis for antigen receptor gene rearrangements in caninelymphoma patients. J Vet Med Sci. 2016 Jun 1;78(5):877-881.

Minimal residual disease in canine lymphoma: An objectivemarker to assess tumour cell burden in remission. Vet J. 2016 Sep;215:38-42.

Intranuclear inclusions in a dog with B-cell leukemia. Vet Clin Pathol. 2016 Dec;45(4):579-583.

Plasma matrix metalloproteinase-9 activity in cats with lymphoma. Vet Comp Oncol. 2017 Mar;15(1):1-5.

Prognostic value of CD44 variant isoform expression in dogs with multicentric high-grade B-cell lymphoma. Am J Vet Res. 2018 Sep;79(9):961-969.

Clinical development